Metabolic characterization of the bovine blastocyst, inner cell mass, trophectoderm and blastocoel fluid
نویسندگان
چکیده
منابع مشابه
Interaction between inner cell mass and trophectoderm of the mouse blastocyst. II. The fate of the polar trophectoderm.
Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the inner cell mass are the oldest mural cells. However, after implant...
متن کاملPrimary differentiation in the human blastocyst: comparative molecular portraits of inner cell mass and trophectoderm cells.
The primary differentiation event during mammalian development occurs at the blastocyst stage and leads to the delineation of the inner cell mass (ICM) and the trophectoderm (TE). We provide the first global mRNA expression data from immunosurgically dissected ICM cells, TE cells, and intact human blastocysts. Using a cDNA microarray composed of 15,529 cDNAs from known and novel genes, we ident...
متن کاملInteraction between inner cell mass and trophectoderm of the mouse blastocyst
Increase in cell number has been compared with mitotic activity in the polar and mural trophectoderm and in the inner cell mass (ICM) of mouse blastocysts. The results indicate that whereas ICM cells divide at a rate which is compatible with the rate of increase of ICM cell number, polar trophectoderm cells divide faster and mural trophectoderm cells slower than can account for their own rates ...
متن کاملDo trophectoderm and inner cell mass cells in the mouse blastocyst maintain discrete lineages?
The extent to which trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst remain distinct during the period from the commencement of cavitation up until 48 h later in culture was investigated. Fluorescent latex microparticles were used to label exclusively all TE cells in nascent blastocysts and the position of labelled progeny in cultured blastocysts was examined by dis...
متن کاملIsolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst.
A bovine trophectoderm cell line was established from a parthenogenetic in vitro-produced blastocyst. To initiate the cell line, 8-day parthenogenetic blastocysts were attached to a feeder layer of STO fibroblasts and primary outgrowths occurred that consisted of trophectoderm, endoderm, and very occasionally epiblast tissue. Any endoderm and epiblast outgrowths were removed from the primary cu...
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ژورنال
عنوان ژورنال: Reproduction
سال: 2003
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep.0.1260299